cd142 anti htf  (R&D Systems)

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting

doi: 10.1002/rth2.12363

Figure Lengend Snippet: Anti‐human and anti‐mouse tissue factor antibodies that were assessed in the present study

Article Snippet: Human , Mouse , Monoclonal , Purified Mouse Anti‐Human CD142 (HTF‐1) , BD Biosciences , 550252 , 6357515 , 0.5 mg/mL , FCM, Inh , Y‐Human.

Techniques: Purification, Coagulation, Recombinant

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting

doi: 10.1002/rth2.12363

Figure Lengend Snippet: Detection of human tissue factor by western blotting using commercial antibodies. We used a tissue factor (TF)‐positive cell line (HPAF‐II) and a TF‐negative cell line (MIA PaCa‐2). Each primary antibody was diluted 1:1000. The final concentration of each antibody was as follows: Novus Biologicals (final concentration 0.64 μg/mL); Cell Signaling Technologies (final concentration 0.055 μg/mL), HTF‐1 (final concentration 0.55 μg/mL), in‐house TF9‐10H10 (final concentration 1.0 μg/mL), Bio‐Rad Laboratories TF9‐10H10 (final concentration 0.055 μg/mL). Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) was detected using an anti‐human GAPDH antibody (1:1000 dilution, final concentration 0.2 μg/mL). Secondary antibodies were used at a 1:10 000 dilution in blocking buffer. A protein marker is shown on the left of each panel

Article Snippet: Human , Mouse , Monoclonal , Purified Mouse Anti‐Human CD142 (HTF‐1) , BD Biosciences , 550252 , 6357515 , 0.5 mg/mL , FCM, Inh , Y‐Human.

Techniques: Western Blot, Concentration Assay, Blocking Assay, Marker